Journal: Bioengineering

Article Title: Highly Porous Type II Collagen-Containing Scaffolds for Enhanced Cartilage Repair with Reduced Hypertrophic Cartilage Formation

doi: 10.3390/bioengineering9060232

Figure Lengend Snippet: The incorporation of CII was associated with a reduction of COLX synthesis, and in combination with HyA, was found to increase the accumulation of COLII in the matrices by day 28. The expression of COLII ( A ), COLX ( B ) and GAPDH (housekeeper) ( C ) proteins was determined for scaffolds seeded with human MSCs from donors 1, donor 2 and donor 3 after 28 days in culture. COLII and COLX protein expression was also determined on cell-free scaffolds ( D ). The relative protein-band intensity of COLII and COLX was quantified and normalised to GAPDH ( E ).

Article Snippet: The membrane was incubated at 4 °C overnight with a 1:1000 solution of diluted rabbit monoclonal [EPR12268] anti-type II collagen (COLII) antibody (Abcam, ab188570), 1:1000 diluted rabbit polyclonal anti-type X collagen (COLX) antibody (Abcam, ab58632) and 1:5000 rabbit polyclonal anti-GAPDH (GAPDH) antibody (Abcam, ab9485).

Techniques: Expressing

Journal: Journal of Orthopaedic Translation

Article Title: Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats

doi: 10.1016/j.jot.2021.06.003

Figure Lengend Snippet: Celecoxib reduces type X collagen, MMP13 and PTHrP levels and inhibits type II collagen expression in osteoarthritic articular cartilage (A) Immunohistochemical analysis of type X collagen (scale bars: 100 ​μm) (B) MMP13 (scale bars: 200 ​μm) (C) type II collagen, and (D) PTHrP (scale bars: 100 ​μm) in articular cartilage of tibia sections in the OA ​+ ​Saline and OA ​+ ​Celec groups. The histogram illustrates the quantification of the positively stained type X collagen and the relative density of MMP13, type II collagen and PTHrP expression in articular cartilage. Each column represents the mean ​± ​SEM of six samples. Data from each group were compared with those of the OA contralateral control group and were evaluated by one-way ANOVA and Scheffe's test. ∗, P ​< ​0.05; ∗∗, P ​< ​0.01; the OA ​+ ​Celec groups compared to the OA ​+ ​Saline groups # , P ​< ​0.05; ## , P ​< ​0.01.

Article Snippet: Sections were then blocked with 10% FBS for 1 ​h and incubated with primary antibodies against type II collagen (1:2000; mouse monoclonal antibody; Chemi-Con, Temecula, CA), type X collagen (rat polyclonal antibody) (1:200; Cosmo Bio, Tokyo, Japan), COX-2 (rabbit polyclonal antibody) (1:50; Santa Cruz, Europe), PTHrP (rabbit polyclonal antibody) (1:100; OriGene, MD, USA) and MMP13 (1:200; rabbit polyclonal antibody) (1:100; Bioss, MA, USA) at 4 ​°C overnight.

Techniques: Expressing, Immunohistochemical staining, Staining